Neutral Protease (Dispase)

<strong>Neutral Protease (Dispase)</strong>_x000D_ <strong>Catalog number:</strong> B2018270_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 5 mg_x000D_ <strong>Molecular Weight or Concentration:</strong> 36 kDa_x000D_ <strong>Supplied as:</strong> Powder_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> 2-8°C_x000D_ <strong>Keywords:</strong> Dispase_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Stenn KS, Link R, Moellmann G, Madri J, Kuklinska E. Dispase, a neutral protease from Bacillus polymyxa, is a powerful fibronectinase and type IV collagenase J Invest Dermatol. 1989 Aug;93(2):287-90._x000D_ 2: Nagata M, Matsumura T. Action of the bacterial neutral protease, dispase, on cultured cells and its application to fluid suspension culture with a review on biomedical application of this protease Jpn J Exp Med. 1986 Dec;56(6):297-307._x000D_ 3: Umbach JA, Gundersen CB, Baker PF. Giant synaptosomes Nature. 1984 Oct 4-10;311(5985):474-7._x000D_ 4: O'Gorman D, Kin T, Pawlick R, Imes S, Senior PA, Shapiro AM. Clinical islet isolation outcomes with a highly purified neutral protease for pancreas dissociation Islets. 2013 May-Jun;5(3):111-5._x000D_ 5: Craviso GL. Generation of functionally competent single bovine adrenal chromaffin cells from cell aggregates using the neutral protease dispase J Neurosci Methods. 2004 Aug 30;137(2):275-81._x000D_ 6: Fiebig D, Storka J, Roeder M, Meyners C, Schmelz S, Blankenfeldt W, Scrima A, Kolmar H, Fuchsbauer HL. Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis-inducing protein from Streptomyces mobaraensis DSM 40487 FEBS J. 2018 Nov;285(22):4246-4264._x000D_ 7: Fujita T, Shiota K, Yoshikawa J, Ogawa S, Aoyagi H. Simple method for analyzing the purity of protease-containing samples by acid-treatment SDS-PAGE J Biosci Bioeng. 2019 Nov;128(5):630-635._x000D_ 8: Kitano Y, Okada N. Separation of the epidermal sheet by dispase Br J Dermatol. 1983 May;108(5):555-60._x000D_ 9: Geggel HS, Gipson IK. Removal of viable sheets of conjunctival epithelium with dispase II Invest Ophthalmol Vis Sci. 1985 Jan;26(1):15-22._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/6784935">10: Cassiman JJ, Brugmans M, Van den Berghe H. Growth and surface properties of dispase dissociated human fibroblasts Cell Biol Int Rep. 1981 Feb;5(2):125-32. </a>_x000D_ _x000D_ <strong>Products Related to Neutral Protease (Dispase) can be found at</strong> <a href="https://moleculardepot.com/product-category/Proteins/"> Proteins</a>