Anti-Excitatory Amino Acid Transporter 1 Antibody

Mouse Monoclonal Antibody specific to Excitatory Amino Acid Transporter 1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Glial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 1, GLAST, GLAST-1, Solute carrier family 1 member 3

Gene Name

Slc1a3

NCBI Gene ID

UniProt

P24942

Cellular Locus

Cell membrane, Multi-pass membrane protein.

Host

Mouse

Reactivity

Rat

Immunogen

Synthetic peptide derived from the C-terminal domain of rat EAAT1. Accession no. P24942.

Target Antigen

Excitatory amino acid transporter 1

Target

Excitatory Amino Acid Transporter 1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ELISA

Field of Research

Neuroscience

Purification Method

Purified by Protein A affinity chromatography

Dilution

Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

Format

Purified

Form

Lyophilized

Buffer

Tris Buffer

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:1279699, PubMed:8387171). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). {UniProtKB:O57321, UniProtKB:P56564, PubMed:1279699, PubMed:8387171}.

Additionnal Information

ELISA: use at 0.1-2ug/ml Immunoblotting: User should use at 1-5ug/ml. A band of ~60kDa is detected. Immunohistochemistry: Use at 2- 10ug/ml. These are recommended concentrations. End user should determine optimal concentrations for their applications.

Storage Conditions

This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody reacts with rat EAAT1 protein. Cross-reactivity with human and mouse EAAT1 is predicted based on sequence homology.

Formulation

Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.

Target Background

Excitatory amino-acid transporters (EAATs), also known as glutamate transporters, belong to the family of neurotransmitter transporters. EAATs serve to terminate the excitatory signal by removal of glutamate from the neuronal synapse into neuroglia and neurons. After glutamate is released as the result of an action potential, glutamate transporters quickly remove it from the extracellular space to keep its levels low, thereby terminating the synaptic transmission. The activity of glutamate transporters also allows glutamate to be recycled for repeated release. Glutamate transporters also transport aspartate and are present in virtually all peripheral tissue. They exhibit stereoselectivity for L-glutamate but transport both L- and D-aspartate.