Biotinylated Human PD-L2 / B7-DC Protein, Avitag™, His Tag (recommended for biopanning)

Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Product Specifications

Background

Specifications

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.2 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAI13679

Expression Region

Leu 20 - Pro 219

Host

HEK293

Target

PD-L2

Conjugation

Biotin-labeled

Tag

C-Avi & C-6xHis

Source

Human

Applications

PD2-H82E8 works best for experiments that test the binding between PD-L2 and candidate antibodies, such as biopanning and other relevant assays. This product is NOT suitable for testing PD1-PDL2 binding. For this type of application, we strongly recommend you to choose PD2-H82F6 as an alternative.

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Anti-PD-L2 MAb (human IgG1) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Avitag,His Tag (Cat. No. PD2-H82E8) with a linear range of 2-50 ng/mL (QC tested).