Fructose-6-Phosphate (F-6-P) Fluorometric Assay Kit
Product Specifications
Type
Fluorescence method
Field of Research
Glycolysis and Lipid Metabolism
Assay Principle
Fructose-6-phosphate (F-6-P) is a six-carbon sugar formed by esterification of fructose and phosphate. It is an important intermediate in glycolysis and gluconeogenesis pathways. As the most basic metabolic substances in the metabolic process of living bodies and cells, it is of great significance to study them quantitatively. Enzyme catalyze F-6-P to produce a specific product, which can react with chromogenic agent to produce the fluorescent substance. Detect the fluorescent intensity of fluorescent substance at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Assay Performance Time
40 min
Sample Type
Animal and plant tissue; Cell
Detection Range
1.06-35.00 μmol/L
Sensitivity
1.06 μmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
5.7-9.8%
Intra CV
4.6-5.0%
Detection Instrument
Fluorescence microplate reader
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