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Fructose-6-Phosphate (F-6-P) Fluorometric Assay Kit

Product Specifications

Type

Fluorescence method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Fructose-6-phosphate (F-6-P) is a six-carbon sugar formed by esterification of fructose and phosphate. It is an important intermediate in glycolysis and gluconeogenesis pathways. As the most basic metabolic substances in the metabolic process of living bodies and cells, it is of great significance to study them quantitatively. Enzyme catalyze F-6-P to produce a specific product, which can react with chromogenic agent to produce the fluorescent substance. Detect the fluorescent intensity of fluorescent substance at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.

Assay Performance Time

40 min

Sample Type

Animal and plant tissue; Cell

Detection Range

1.06-35.00 μmol/L

Sensitivity

1.06 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

5.7-9.8%

Intra CV

4.6-5.0%

Detection Instrument

Fluorescence microplate reader

Frequently Asked Questions

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