5 (6) -Carboxy-2',7'-dichlorofluorescein diacetate

5 (6) -Carboxy-2',7'-dichlorofluorescein diacetate is a cell permeant fluorescent indicator. 5 (6) -Carboxy-2',7'-dichlorofluorescein diacetate can be used to assess reactive oxygen species (ROS) generation within human neuronal-glial (HNG) cells in primary co-culture[1]

Product Specifications

CAS Number

[127770-45-0]

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Reactive Oxygen Species (ROS)

Type

Dye Reagents

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/5-6-carboxy-2-7-dichlorofluorescein-diacetate.html

Purity

97.5

Solubility

DMSO : 125 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

ClC1=C(OC(C)=O)C=C2OC3=CC(OC(C)=O)=C(Cl)C=C3C(OC4=O)(C5=CC=C(C(O)=O)C=C54)C2=C1.ClC6=C(OC(C)=O)C=C7OC8=CC(OC(C)=O)=C(Cl)C=C8C(OC9=O)(C%10=CC(C(O)=O)=CC=C%109)C7=C6

Molecular Formula

C25H14Cl2O9

Molecular Weight

529.28

Precautions

H315, H319, H335

References & Citations

[1]Pogue AI, et al. Metal-sulfate induced generation of ROS in human brain cells: detection using an isomeric mixture of 5- and 6-carboxy-2',7'-dichlorofluorescein diacetate (carboxy-DCFDA) as a cell permeant tracer. Int J Mol Sci. 2012;13 (8) :9615-26.