Anti-Measles Phosphoprotein Antibody

Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

ELISA: use at 0.1-1.0ug/ml <br><br>Immunoblotting: <br><br>User should use at 1-5ug/ml. A band of ~70kDa is detected <br><br>Immunocytochemistry: use at 2- 10ug/ml on virus-infected cells. <br><br>These are recommended concentrations. <br><br>End user should determine optimal concentrations for their applications.

This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Measles virus (MeV) is an enveloped RNA virus in the Morbillivirus genus of the Paramyxoviridae family. Its non- segmented, negative-sense, single- stranded RNA genome is encapsidated by the viral nucleoprotein (N) within a helical nucleocapsid. Transcription and replication are carried out on this N:RNA complex by the viral polymerase complex which consists of two components, the large protein (L) and the phosphoprotein (P). MeV N consists of two regions: a structured N-terminal moiety, NCORE (aa 1–400), which contains all the regions necessary for self-assembly and RNA-binding, and a C-terminal domain, NTAIL(aa 401–525) that is intrinsically unstructured and is exposed at the surface of the viral nucleocapsid.