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Anti-CREB (phospho-Ser133) Antibody

Rabbit Polyclonal Antibody specific to CREB (phospho-Ser133)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-CREB (phospho-Ser133) Rabbit Polyclonal Antibody

UniProt

P16220

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of Serine 133 (R-P-S(p)-Y-R) derived from human CREB and conjugated to KLH.

Target

CREB (phospho-Ser133)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Western blotting: use at dilution of 1:500- 1:1,000. A band of ~43kDa is detected. <br><br>Immunohistochemistry: use at dilution of 1:50- 1:100. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat CREB only when phosphorylated at serine 133.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

CREB (cAMP response element-binding protein) is a cellular transcription factor. It binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the downstream genes. Genes whose transcription is regulated by CREB include: c-fos, BDNF, tyrosine hydroxylase, numerous neuropeptides (such as somatostatin, enkephalin, VGF, corticotropin-releasing hormone), and genes involved in the mammalian circadian clock (PER1, PER2).

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