Anti-Cav3.2 Ca+2 Channel Antibody

Mouse Monoclonal Antibody specific to Cav3.2 Ca +2 Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Low-voltage-activated calcium channel α1 3.2 subunit, Voltage-gated calcium channel subunit α Cav3.2

Gene Name

CACNA1H

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=CACNA1H">CACNA1H</a>

UniProt

O95180

Accession Number

NP_001005407.1

Cellular Locus

Cell membrane

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1019-1293 (II-III loop)

Target Antigen

Voltage-dependent T-type calcium channel subunit α-1H

Target

Cav3.2 Ca+2 Channel

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, AM

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Voltage-sensitive calcium channel that gives rise to T-type calcium currents. T-type calcium channels belong to the 'low-voltage activated (LVA)' group. A particularity of this type of channel is an opening at quite negative potentials, and a voltage-dependent inactivation (PubMed:9670923, PubMed:9930755, PubMed:27149520). T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle (Probable). They may also be involved in the modulation of firing patterns of neurons (PubMed:15048902). In the adrenal zona glomerulosa, participates in the signaling pathway leading to aldosterone production in response to either AGT/angiotensin II, or hyperkalemia (PubMed:25907736, PubMed:27729216). {PubMed:24277868, PubMed:25907736, PubMed:27149520, PubMed:27729216, PubMed:9670923, PubMed:9930755, PubMed:15048902}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of >260kDa is detected.Immunohistochemistry and and Immunocytochemistry: use at 0.1-1ug/mL. Immunofluorescence: use at 1-10ug/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain membranes.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Cav3.2. It does not cross-react with Cav1.3.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cav3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest that certain mutations in this gene lead to childhood absence epilepsy. It has also been suggested that up-regulation of Cav3.2 may be involved in the progression of prostate cancer toward an androgen -independent stage.