EN219-alkyne

EN219-alkyne is an alkyne-functionalized EN219 probe. EN219 (HY-P0287A) is a moderately selective synthetic covalent ligand against an N-terminal cysteine (C8) of RNF114 with an IC50 of 470 nM. EN219 inhibits RNF114-mediated autoubiquitination and p21 ubiquitination[1][2]. EN219-alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Product Specifications

Product Name Alternative

2'3'-didehydro-2'3'-dideoxyadenosine

UNSPSC

12352005

Target

Fluorescent Dye

Type

Reference compound

Related Pathways

Others

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/en219-alkyne.html

Concentration

10mM

Purity

99.62

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

C#CCNC(COC1=CC=C(C2CC(C3=CC=C(Br)C=C3)=NN2C(CCl)=O)C=C1)=O

Molecular Formula

C22H19BrClN3O3

Molecular Weight

488.76

References & Citations

[1]Luo M, et al. Chemoproteomics-enabled discovery of covalent RNF114-based degraders that mimic natural product function. Cell Chem Biol. 2021 Apr 15;28 (4) :559-566.e15.