Anti-Caspase-12 (NT) Antibody

Rabbit Polyclonal Antibody specific to Caspase-12 (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CASP-12, EC 3.4.22.-

Gene Name

Casp12

Gene ID

12364

NCBI Gene ID

UniProt

O08736

Accession Number

NP_033938.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 2-17 at the N-terminus of mouse caspase-12 (accession no. CAA73532).

Target Antigen

Caspase-12

Target

Caspase-12 (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. {ECO:0000250}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunohistochemistry: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Tissue lysate of mouse brain.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat caspase-12 (approx. 53kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Three distinct signaling pathways lead to programmed cell death. The death receptor and mitochondrial pathways are the main pathways in which the proteases caspase-8 and caspase- 9, respectively, are involved. Endoplasmic reticulum (ER) stress is the third pathway, and caspase-12 is involved. Caspase-12, localized in the ER, is activated by ER stress and mediates ER stress-induced apoptosis. Several proteins that are associated with Alzheimer's disease, including -secretase, presenilin, and â€“ amyloid precursor protein (APP), co-localize in the ER with caspase-12. Caspase-12 is ubiquitously expressed in mouse tissues.