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SingleFlowEx CD79a PE

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

HM57

Applications

FC (QC tested)

Concentration

0.070 mg/mL

Format

PE

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al: CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood. 1995 Aug 15;86 (4) :1453-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/7632952?ordinal, *Jones M, Cordell JL, Beyers AD, Tse AG, Mason DY: Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J Immunol. 1993 Jun 15;150 (12) :5429-35., URL: http://www.ncbi.nlm.nih.gov/pubmed/8515069?ordinal, *van Noesel CJ, van Lier RA, Cordell JL, Tse AG, van Schijndel GM, de Vries EF, Mason DY, Borst J: The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. J Immunol. 1991 Jun 1;146 (11) :3881-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/2033258?ordinal, *Mason DY, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen JJ: The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation. Eur J Immunol. 1992 Oct;22 (10) :2753-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/1396979?ordinal, *Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119 (1-2) :56-62. , URL: http://www.ncbi.nlm.nih.gov/pubmed/17673300 , *Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147 (11) :2474-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/1747162?ordinal, *Faldyna M, Sinkora J, Knotigova P, Rehakova Z, Moravkova A, Toman M: Flow cytometric analysis of bone marrow leukocytes in neonatal dogs. Vet Immunol Immunopathol. 2003 Oct 15;95 (3-4) :165-76. doi: 10.1016/s0165-2427 (03) 00135-1. (dog), URL: https://pubmed.ncbi.nlm.nih.gov/12963277/, *Use of this product has not been published., URL:

Other References

*Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a) - and Igbeta (CD79b) -mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177 (11) :7913-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/17114463?ordina, *Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174 (3) :1245-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/15661879?ordina, *Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173 (2) :1000-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/15240688?ordina, *Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172 (4) :2210-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/14764688?ordina, *Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194 (11) :1583-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/11733573?ordina

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

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