Recombinant Human Rock-2 Protein (His Tag)
Product Specifications
Background
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Abbreviation
Rock-2
Synonyms
ROCK 2; ROCK2; ROK alpha; ROKalpha; Rho kinase 2; Rho-associated; Rho-associated protein kinase 2; Rock II; Rock2; Rock2m
UniProt
O75116
Accession Number
O75116
Expression System
E.coli
Tag
N-Trx
Sequence
Thr1212-Ser1388
Field of Research
Cell Biology; Cardiovascular; Signal Transduction
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
39.3 kDa
Observed Molecular Weight
39 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
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