Cynomolgus Siglec-10 Protein, His Tag (MALS verified)

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.2 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

A0A2K5WBX8-1

Expression Region

Thr 17 - Ala 549

Host

HEK293

Target

Siglec-10

Conjugation

Unconjugated

Tag

C-10xHis

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Cynomolgus Siglec-10, His Tag (Cat. No. SI0-C52H5) at 1 μg/mL (100 μL/well) on an Nickel Coated plate can bind Anti-Siglec-10 Antibody, Human IgG1 with a linear range of 0.009-0.156 μg/mL (QC tested).