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Anti-p53R2 (NT) Antibody

Rabbit Polyclonal Antibody specific to p53R2 (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 1.17.4.1, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2

Gene Name

RRM2B

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=RRM2B">RRM2B</a>

UniProt

Q7LG56

Cellular Locus

Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus in response to DNA damage.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 2-17 of human p53R2.

Target Antigen

Ribonucleoside-diphosphate reductase subunit M2 B

Target

p53R2 (NT)

Clonality

Polyclonal

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Cancer research

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. {PubMed:10716435, PubMed:11517226, PubMed:11719458}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~39kDa is detected <br><br>IHC: use at 1ug/mL. <br><br>Immunofluorescence: use at 20ugml.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes an epitope at the N-terminus of human, mouse, and rat p53R2.

Formulation

PBS, pH 7.4, 0.02% sodium azide.

Buffer pH

pH 7.4

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