Anti-IRAK-4 Antibody

Rabbit Polyclonal Antibody specific to IRAK-4

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

IRAK-4, EC 2.7.11.1, Renal carcinoma antigen NY-REN-64

Gene Name

IRAK4

NCBI Gene ID

UniProt

Q9NWZ3

Cellular Locus

Cytoplasm

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4.

Target Antigen

Interleukin-1 receptor-associated kinase 4

Target

IRAK-4

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. {PubMed:11960013, PubMed:12538665, PubMed:15084582, PubMed:17217339, PubMed:17337443, PubMed:17878374, PubMed:17997719, PubMed:20400509, PubMed:24316379}.

Additionnal Information

Immunoblotting: Use at 1-4ug/mL. A band of approximately 50kDa is detected. Immunocytochemistry: Use at 10ug/mL. Immunofluorescence: Use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

Stable for one year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

This antibody specifically recognizes human IRAK-4 (IL-1 Receptor Associated Kinase-4). This antibody does not cross-react with other IRAKs.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

IRAK-4 activates the NF-ï«B and MAPK pathways and plays a role in IL-1R mediated inflammatory responses and innate immunity. IRAK-4-deficient animals are resistant to challenge with LPS and are impaired in their responses to viral and bacterial challenges.