Biotinylated Human HLA-A*33:03&B2M Monomer Protein (Peptide free, MALS verified)

Biotinylated Human HLA-A*33:03&B2M Monomer Protein (HLM-H82W2) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*03:03) & Ile 21 - Met 119 (B2M) (Accession # AAA79865.1 (HLA-A*03:03) & P61769-1 (B2M) ) .

Product Specifications

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 39.4 kDa and 15.7 kDa. The protein migrates as 43-48 kDa and 16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA79865.1 (HLA-A*03:03) & P61769-1 (B2M)

Expression Region

Gly 25 - Thr 305 (HLA-A*33:03) & Ile 21 - Met 119 (B2M)

Host

HEK293

Target

HLA-A*3303 & B2M

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human HLA-A*33:03&B2M Monomer Protein (Cat. No. HLM-H82W2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.02-2 ng/mL (QC tested).