Recombinant Human Vascular endothelial growth factor A, long form (VEGFA) (Active)
Product Specifications
Product Name Alternative
Vascular Endothelial Growth Factor Isoform 165; VEGF165
Abbreviation
Recombinant Human VEGFA protein (Active)
Gene Name
VEGFA
UniProt
P15692-4
Expression Region
27-191aa
Organism
Homo sapiens (Human)
Target Sequence
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Tag
C-terminal 6xHis-tagged
Type
Active Protein & In Stock Protein
Source
Mammalian cell
Field of Research
Others
Endotoxin
≤10 EU/mg by the LAL method
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilic vein endothelial cells. The ED50 for this effect is 0.2-10 ng/mL.
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
20 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein of Isoform VEGF165
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