DTNB

DTNB (Ellman’s Reagent) is a chemical used to quantify the number or concentration of thiol groups[1].

Product Specifications

CAS Number

[69-78-3]

Product Name Alternative

Ellman’s Reagent

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/DTNB.html

Purity

99.90

Solubility

DMSO : 100 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : < 0.1 mg/mL (ultrasonic)

Smiles

O=C(O)C1=C([N+]([O-])=O)C=CC(SSC2=CC(C(O)=O)=C([N+]([O-])=O)C=C2)=C1

Molecular Formula

C14H8N2O8S2

Molecular Weight

396.35

Precautions

H302, H315, H319, H335

References & Citations

[1]T Nagaoka, et al. DTNB modification of SH groups of isocitrate dehydrogenase from Bacillus stearothermophilus purified by affinity chromatography. J Biochem. 1977 Jan;81 (1) :71-8.|[2]Sullivan ML, et al. Spectrophotometric determination of reaction rates and kinetic parameters of a BAHD acyltransferase using DTNB (5,5'-dithio-bis-[2-nitrobenzoic acid]) . Plant Sci. 2018 Apr;269:148-152.