Anti-JunD (Phospho-Ser255) Antibody

Rabbit Polyclonal Antibody specific to JunD (Phospho-Ser255)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-JunD (Phospho-Ser255) Rabbit Polyclonal Antibody

Gene Name

JUND

NCBI Gene ID

UniProt

P17535

Cellular Locus

Nucleus.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of serine 255 (G-E-S(p)-P-P) derived from human JunD and conjugated to KLH.

Target Antigen

Transcription factor JunD

Target

JunD (Phospho-Ser255)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:9989505, PubMed:28981703). {PubMed:28981703, PubMed:9989505}.

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~38kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat JunD only when phosphorylated at serine 255.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

JunD protein encoded by this intronless gene is a member of the JUN family and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms.