TPE-MI

TPE-MI (Tetraphenylethene maleimide) is a thiol probe for measuring unfolded protein load and proteostasis in cells (the excitation wavelength is 350 nm and the emission wavelength is 470 nm) . TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin research of the malaria parasitesPlasmodium falciparum [1][2].

Product Specifications

CAS Number

[1245606-71-6]

Product Name Alternative

Tetraphenylethene maleimide

UNSPSC

12171500

Hazard Statement

H302, H315, H319

Target

Huntingtin; Parasite

Type

Dye Reagents

Related Pathways

Anti-infection; Neuronal Signaling

Applications

Neuroscience-Neurodegeneration

Field of Research

Infection; Neurological Disease

Assay Protocol

https://www.medchemexpress.com/tpe-mi.html

Purity

98.93

Solubility

DMSO : 8.57 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=C(C=C1)N(C2=CC=C(/C(C3=CC=CC=C3)=C(C4=CC=C(C)C=C4)/C5=CC=CC=C5)C=C2)C1=O

Molecular Formula

C31H23NO2

Molecular Weight

441.52

Precautions

H302, H315, H319

References & Citations

[1]Chen MZ, et al. A thiol probe for measuring unfolded protein load and proteostasis in cells. Nat Commun. 2017;8 (1) :474. Published 2017 Sep 7. |[2]Hu Q, et al. In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe. Molecules. 2021;26 (14) :4273. Published 2021 Jul 14.