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Recombinant Chicken Growth/differentiation factor 9 (GDF-9)

Product Specifications

Abbreviation

Recombinant Chicken GDF-9 protein

Gene Name

GDF-9

UniProt

Q6Q247

Expression Region

25-454aa

Organism

Gallus gallus (Chicken)

Target Sequence

SPRSRDRTAPDKVSGLLGAPELHPLLRLPKGVSRGYALLPPLLEVLSDQGRQSWESGTPRLQPDSRALRYMKRLYKMYATKEGIPKAHKSHLYNTVRLFTPCSECQHRHRDPITGDFHSVDLLFNLDRVTALEHLLKSVLLYSFDTSVPTSSFTCTCHLSVKEHDFSSQVCPSISHSVAFSLHFEVRKRKWVEIDVTSFLRPLIATNRRNIHMAVNFTCLMGNPQHNTKQDNLINVALVPPSLLLYLNDTSEQAYHRWNSLRHRRKSPVRPKQRSSLFADVTGDEGRQNTQGKRASRHRREENLKEAPATAPQNLSEYFKQFLFPQNECELHSFRLSFSQLKWDKWIIAPHRYSPQYCKGDCPRVVGHRYGSPVHTMVQNIIYEKLDSSVPRPSCVPAEYSPLSVLTIEPDGSIVYKEYEDMIATKCTCR

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

56.8 kDa

References & Citations

"Structural basis for substrate selectivity in human maltase-glucoamylase and sucrase-isomaltase N-terminal domains." Sim L., Willemsma C., Mohan S., Naim H.Y., Pinto B.M., Rose D.R. J. Biol. Chem. 285:17763-17770 (2010)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length of Mature Protein

Available Sizes

Frequently Asked Questions

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