Human SLC7A11&SLC3A2 Full Length Protein, Flag, His Tag (Detergent)

Cysteine plays an essential role in cellular redox homoeostasis as a key constituent of the tripeptide glutathione (GSH) . A rate limiting step in cellular GSH synthesis is the availability of cysteine. However, circulating cysteine exists in the blood as the oxidised di-peptide cystine, requiring specialised transport systems for its import into the cell. System xc- is a dedicated cystine transporter, importing cystine in exchange for intracellular glutamate. To counteract elevated levels of reactive oxygen species in cancerous cells system xc- is frequently upregulated, making it an attractive target for anticancer therapies.

Product Specifications

Background

Specifications

The SLC7A11 protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. The SLC3A2 protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 59.4 kDa & 69.9 kDa.

Accession Number

Q9UPY5 & P08195

Expression Region

Val 2 - Leu 501 & Glu 2 - Ala 630

Host

HEK293

Target

SLC7A11 & SLC3A2

Conjugation

Unconjugated

Tag

N-Flag & C-10xHis & N-10xHis

Source

Human & Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Bioactivity

Immobilized Human SLC7A11&SLC3A2 Full Length Protein, Flag,His Tag (Cat. No. SLS-H52D3) at 5 μg/mL (100 μL/well) can bind Anti-SLC7A11 Antibody with a linear range of 2-63 ng/mL (QC tested).