Donkey Chicken IgY, conjugated with Agarose

A donkey was immunized with purified chicken IgY. Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.

The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 mL PBS (0.1 mg/mL) to 1.0 mL bed volume of agarose. Two mL aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per mL agarose.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

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