Anti-Mre11 Antibody

Mouse Monoclonal Antibody specific to Mre11

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.1.-.-, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog A

Gene Name

MRE11

NCBI Gene ID

UniProt

P49959

Cellular Locus

Nucleus, Chromosome, telomere, Chromosome

Host

Mouse

Reactivity

Human

Immunogen

GST fusion protein corresponding to aa 182-582 of Mre11.

Target Antigen

Double-strand break repair protein MRE11

Target

Mre11

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IHC-P, IP, ELISA, Functional Assay, PLA

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:29670289, PubMed:30612738, PubMed:9590181, PubMed:9705271}.

Additionnal Information

Immunoblotting: Use at 0.5-2ug/mL. A band of ~80kDa is detected. Detection of Mre11 in 30ug of whole cell extracts. Immunofluorescence: use at 1-5ug/mL. Detection of Mre11 in HeLa cells fixed in 4% paraformaldehyde for 15 minutes. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C to-70°C. Store as concentrated solution in appropriate aliquots to avoid multiple freeze-thaw cycles.