Human IL-8 / CXCL8 Protein, premium grade
Human IL-8 Protein, premium grade (IL8-H5217) is expressed from human 293 cells (HEK293) . It contains AA Ser 28 - Ser 99 (Accession # P10145-1) .
Product Specifications
Background
Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2) . IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively.
Specifications
This protein carries no "tag". The protein has a calculated MW of 8.4 kDa. The protein migrates as 9 kDa±3 kDa under reducing (R) condition, and 11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Accession Number
P10145-1
Expression Region
Ser 28 - Ser 99
Host
HEK293
Target
IL-8
Conjugation
Unconjugated
Tag
Native
Source
Human
Stability
● -20°C to -70°C for 24 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.01 EU per μg
Purity
95%
Bioactivity
Immobilized Human IL-8 Protein, premium grade (Cat. No. IL8-H5217) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL-8 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
8.4 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
200ug*5
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/IL8-H5217.pdf
Species
Human
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