Anti-Glutathione-S-Transferase (GST) Antibody
Selected Variant
18844-1
Size: 1 mg
Rabbit Polyclonal Antibody specific to Glutathione-S-transferase (GST)
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
GST 28
UniProt
P26624
Host
Rabbit
Reactivity
Glutathione-S-Transferase (Gst)
Immunogen
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Target Antigen
Glutathione S-transferase class-mu 28 kDa isozyme
Target
Glutathione-S-Transferase (GST)
Clonality
Polyclonal
Isotype
Whole IgG
Type
Antibody
Applications
WB, IP, ICC, ELISA
Field of Research
Epitope Tags
Purification Method
Purified by affinity on GST
Concentration
1.0 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Additionnal Information
Immunoblotting: use at a dilution of 1:1,000- 1:20,000.
<br><br>Immunocytochemistry: use at a dilution of 1:100-1:400.
<br><br>Immunoprecipitation: use 1-4ug/mg lysate.
<br><br>ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500
<br><br>These are recommended dilutions.
<br><br>Endusers should determine optimal dilutions for their applications.
Storage Conditions
This antibody is stable for at least one (1) year at 2-8°C.
Specificity
Antibody was affinity-purified on GST.
Formulation
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer pH
pH 7.2
Target Background
A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
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