Anti-Glutathione-S-Transferase (GST) Antibody

Rabbit Polyclonal Antibody specific to Glutathione-S-transferase (GST)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GST 28

UniProt

P26624

Host

Rabbit

Reactivity

Glutathione-S-Transferase (Gst)

Immunogen

Glutathione-S-Transferase (GST) from Schistosoma japonicum.

Target Antigen

Glutathione S-transferase class-mu 28 kDa isozyme

Target

Glutathione-S-Transferase (GST)

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IP, ICC, ELISA

Field of Research

Epitope Tags

Purification Method

Purified by affinity on GST

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are recommended dilutions. Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

Antibody was affinity-purified on GST.

Formulation

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide

Buffer pH

pH 7.2

Target Background

A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.