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HIST1H3A Antibody

This Tri-Methyl-Histone H3 (Lys27) Antibody is an unconjugated polyclonal product. It tagets HIST1H3A using a Peptide sequence around Tri-Methylation site of lysine 27 (A-R-K (tri-methyl) -S-A) derived from Human Histone H3 as the immunogen. This antibody is suitable for ELISA, IF, IHC, WB.

Product Specifications

Product Name Alternative

Anti-H3/a antibody, anti- H3/c antibody, anti- H3/d antibody, anti- H3/f antibody, anti- H3/h antibody

UniProt

P68431

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around Tri-Methylation site of lysine 27 (A-R-K (tri-methyl) -S-A) derived from Human Histone H3.

Target

HIST1H3A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy u

Dilution

WB: 1: 500-1: 1000, IHC-P: 1: 50-1: 100, IF: 1: 100-1: 200

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

ELISA, IF, IHC, WB

Host or Source

Rabbit

Preservative

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Isotype

IgG

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