High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)

Product Specifications

Target

HDL-C

Type

Colorimetric method

Field of Research

Metabolic Diseases; Glycolysis and Lipid Metabolism

Assay Principle

CM, VLDL and LDL coagulate in a polyanionic environment to form a polymer and are masked by the polymer. High-density lipoprotein (HDL) form soluble compounds under the action of a surfactant, so that HDL-C can directly react with enzyme reagents containing cholesterol esterase (CE) and cholesterol oxidase (CO) to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by peroxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound, the shade is proportional to the amount of HDL-C.

Assay Performance Time

30 min

Sample Type

Serum; Plasma

Detection Range

0.06-3.8 mmol/L

Sensitivity

0.06 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months with shading light.

Citation 01

https://academic.oup.com/cardiovascres/advance-article-abstract/doi/10.1093/cvr/cvae148/7717207

Citation 02

https://www.sciencedirect.com/science/article/pii/S0048969724039822

Citation 03

https://www.sciencedirect.com/science/article/pii/S2212877824001844

Citation 04

https://www.tandfonline.com/doi/abs/10.2147/IJN.S478589

Citation 05

https://www.sciencedirect.com/science/article/pii/S0944711324005440

Citation 06

https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo03183k

Citation 07

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c03172

Citation 08

https://www.sciencedirect.com/science/article/pii/S0014299923007756

Citation 09

https://www.mdpi.com/2304-8158/13/17/2760

Citation 10

https://www.mdpi.com/1422-0067/25/22/12105

Citation 11

https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1510318/full

Citation 12

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202400412RR

Inter CV

0.0005

Intra CV

0.0003

Detection Instrument

Microplate reader

Documents

Product Datasheet

Click to open

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