Low-density Lipoprotein Cholesterol (LDL-C) Assay Kit

Product Specifications

Target

LDL-C

Type

Colorimetric method

Field of Research

Metabolic Diseases; Glycolysis Lipid Metabolism

Assay Principle

Lipoproteins (except LDL) such as HDL, CM, and VLDL change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color development. At this time, the LDL particles are still intact, and then the reagent containing coupling agent is added, which can dissociate the LDL particles to release cholesterol, which is catalyzed by cholesterol esterase (CE) and cholesterol oxidase (CO) and produce hydrogen peroxide. Hydrogen peroxide is catalyzed by oxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound. Because the cholesterol molecules of other lipoproteins have been removed, the shade is proportional to the amount of LDL-C.

Assay Performance Time

30 min

Sample Type

Serum; Plasma; Cell

Detection Range

0.04-12 mmol/L

Sensitivity

0.04 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2-8°C for 12 months with shading light.

Citation 01

https://academic.oup.com/cardiovascres/advance-article-abstract/doi/10.1093/cvr/cvae148/7717207

Citation 02

https://www.sciencedirect.com/science/article/pii/S2212877824001844

Citation 03

https://www.tandfonline.com/doi/abs/10.2147/IJN.S478589

Citation 04

https://www.sciencedirect.com/science/article/pii/S0944711324005440

Citation 05

https://www.sciencedirect.com/science/article/pii/S094471132400432X

Citation 06

https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo03183k

Citation 07

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c03172

Citation 08

https://www.sciencedirect.com/science/article/pii/S0014299923007756

Citation 09

https://www.mdpi.com/2304-8158/13/17/2760

Citation 10

https://www.sciencedirect.com/science/article/pii/S1567576924011901

Citation 11

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202400412RR

Citation 12

https://www.sciencedirect.com/science/article/pii/S2212429224001755

Inter CV

0.001

Intra CV

0.00055

Detection Instrument

Microplate reader

Documents

Product Datasheet

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