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Human IL-23 alpha & Mouse IL-12 beta Heterodimer (HEK293, His-Avi)

IL-23 alpha and IL-12 beta are the IL-23p19 and IL-12p40 subunits, respectively, composing IL-23 via heterodimerization manner. IL-23 binds to a heterodimeric receptor composed of IL-12RB1 and IL-23R, activates the Jak-Stat signaling cascade, acts on memory CD4 (+) T cells preferentially[1]. IL-23 also involves in activation of several pathways including p38 MAPK or NF-κB and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A[2]. Human IL-23 alpha & Mouse IL-12 beta Heterodimer Protein is produced in HEK293 cells, with a C-Terminal Avi and His-tag.

Product Specifications

Product Name Alternative

Human IL-23 alpha & Mouse IL-12 beta Heterodimer Protein (HEK293, His-Avi), Human; Mouse, HEK293

UNSPSC

12352202

Type

Recombinant Proteins

Assay Protocol

https://www.medchemexpress.com/cytokines/human-il-23a-mouse-il-12b-heterodimer-protein-hek293-his-avi.html

Molecular Formula

51561&16160 (Gene_ID) Q9NPF7 (R20-P189) &P43432 (M23-S335) (Accession)

Molecular Weight

Approximately 22 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta), based on SDS-PAGE under reducing conditions, due to the glycosylation.

References & Citations

[4]Teng MW, et al. IL-12 and IL-23 cytokines: from discovery to targeted therapies for immune-mediated inflammatory diseases. Nat Med. 2015 Jul;21 (7) :719-29.|[5]Li Y, et al. Regulation of Memory T Cells by Interleukin-23. Int Arch Allergy Immunol. 2016;169 (3) :157-62.|[6]Langowski JL, et al. IL-23 promotes tumour incidence and growth. Nature. 2006 Jul 27;442 (7101) :461-5.|[7]Murphy CA, et al. Divergent pro- and antiinflammatory roles for IL-23 and IL-12 in joint autoimmune inflammation. J Exp Med. 2003 Dec 15;198 (12) :1951-7.|[8]Yen D, et al. IL-23 is essential for T cell-mediated colitis and promotes inflammation via IL-17 and IL-6. J Clin Invest. 2006 May;116 (5) :1310-6. |[9]Chan JR, et al. IL-23 stimulates epidermal hyperplasia via TNF and IL-20R2-dependent mechanisms with implications for psoriasis pathogenesis. J Exp Med. 2006 Nov 27;203 (12) :2577-87. |[10]Yago T, et al. IL-23 induces human osteoclastogenesis via IL-17 in vitro, and anti-IL-23 antibody attenuates collagen-induced arthritis in rats. Arthritis Res Ther. 2007;9 (5) :R96. |[1]Oppmann B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity. 2000 Nov;13 (5) :715-25.|[2]Parham C, et al. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R. J Immunol. 2002 Jun 1;168 (11) :5699-708.|[3]Neurath MF. IL-23 in inflammatory bowel diseases and colon cancer. Cytokine Growth Factor Rev. 2019 Feb;45:1-8.

Shipping Conditions

Room temperature in continental US; may vary elsewhere.

Scientific Category

Recombinant Proteins

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