BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R]
Selected Variant
HA601345
Size: 100 µL
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Swiss Prot
P51532 Human, Q3TKT4 Mouse, Q8K1P7 Rat
Cellular Locus
Nucleus.
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within human BRG1 aa 230-280.
Isotype
IgG1
Clone
A5C9-R
Conjugation
Non-conjugated
Type
Recombinant Mouse monoclonal Antibody
Applications
WB
Positive Control
HeLa cell lysate, K-562 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C6 cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 185 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Price
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