Vaccinia Capping Enzyme, GMP Grade

The vaccinia capping enzyme together with substrates, is capable to add the 7-methylguanosine capping structure (m7Gppp, Cap0) to the 5´ end of the RNA. In eukaryotes, this structure is closely related to the stability, transport, and translation of mRNA. Capping RNA with an enzymatic reaction is a simple and effective method that can significantly improve the stability and translation capabilities of RNA used for in vitro transcription, transfection, and micro-injection. The vaccinia virus capping enzyme consists of two subunits (D1 and D12) (Figure 1a), and possesses RNA triphosphatase, guanosine transferase, and guanine methyltransferase activities, and they are all necessary for adding a complete Cap0 structure m7Gppp5´N to mRNA (Figure 1b). This product can be applied to capping reaction of RNA generated by the T7 RNA Polymerase (GMP-E121, Novoprotein). The capping reaction is completed within an hour and the efficiency is close to 100% with correct direction. Vaccinia Capping Enzyme is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing. This product has completed the DMF record of FDA and passed the HALAL certification.