Smad1 (Phospho Ser214) rabbit pAb

Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). PTM: Phosphorylated on serine by BMP type 1 receptor kinase. PTM: Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. similarity: Belongs to the dwarfin/SMAD family. similarity: Contains 1 MH1 (MAD homology 1) domain. similarity: Contains 1 MH2 (MAD homology 2) domain. subcellular location: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. subunit: Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1. tissue specificity: Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Product Specifications

Background

Function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) ., PTM:Phosphorylated on serine by BMP type 1 receptor kinase., PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1., similarity:Belongs to the dwarfin/SMAD family., similarity:Contains 1 MH1 (MAD homology 1) domain., similarity:Contains 1 MH2 (MAD homology 2) domain., subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4., subunit:Interacts with HGS, NANOG and ZCCHC12 (By similarity) . May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1., tissue specificity:Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Product Name Alternative

Mothers against decapentaplegic homolog 1 (MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1)

UniProt

Q15797

Swiss Prot

Q15797

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human Smad1 (Phospho Ser214)

Target

Smad1

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC

Concentration

1 mg/ml

Dilution

WB 1:500-2000; IHC-p 1:50-300

Buffer

-20°C/1 year

Molecular Weight

51kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

51kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271) . Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271) . Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity) . .