8-pCPT-2′-O-Me-cAMP (sodium)

8-pCPT-2′-O-Me-cAMP (8-CPT-2'-O-Me-cAMP) sodium, an analog of cAMP, is an activator of exchange proteins activated by cAMP (Epac) . 8-pCPT-2′-O-Me-cAMP sodium activates Epac1 (EC50 = 2.2 μM), but not PKA (EC50 >10 μM) . 8-pCPT-2′-O-Me-cAMP sodium stimulates Epac-mediated Ca2+ release in pancreatic β-cells in vitro. 8-pCPT-2′-O-Me-cAMP sodium is a Rap1 activator. 8-pCPT-2′-O-Me-cAMP sodium enhances the retinal pigment epithelium barrier against the pathological choroidal endothelial cell invasion that occurs in macular degeneration[1][2][3].

Product Specifications

CAS Number

[634207-53-7]

Product Name Alternative

8-CPT-2'-O-Me-cAMP (sodium)

UNSPSC

12352005

Target

Ras

Type

Reference compound

Related Pathways

GPCR/G Protein; MAPK/ERK Pathway

Applications

Neuroscience-Neuromodulation

Field of Research

Cardiovascular Disease

Assay Protocol

https://www.medchemexpress.com/8-cpt-2me-camp-sodium.html

Purity

99.81

Solubility

DMSO : 125 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

NC1=C2C(N([C@H]3[C@@H]([C@@](O4)([H])[C@](COP4(O[Na])=O)([H])O3)OC)C(SC5=CC=C(Cl)C=C5)=N2)=NC=N1

Molecular Formula

C17H16ClN5NaO6PS

Molecular Weight

507.82

References & Citations

[1]Enserink JM, Christensen AE, de Rooij J, van Triest M, Schwede F, Genieser HG, Døskeland SO, Blank JL, Bos JL. A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK. Nat Cell Biol. 2002 Nov;4 (11) :901-6.|[2]Kang G, Joseph JW, Chepurny OG, Monaco M, Wheeler MB, Bos JL, Schwede F, Genieser HG, Holz GG. Epac-selective cAMP analog 8-pCPT-2'-O-Me-cAMP as a stimulus for Ca2+-induced Ca2+ release and exocytosis in pancreatic beta-cells. J Biol Chem. 2003 Mar 7;278.|[3]Wittchen ES, et al. Rap1 GTPase activation and barrier enhancement in rpe inhibits choroidal neovascularization in vivo. PLoS One. 2013 Sep 10;8 (9) :e73070.