Anti-Chloride Channel Protein 3 (Clcn3) Antibody

Mouse Monoclonal Antibody specific to Chloride Channel Protein 3

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Chloride channel protein 3, ClC-3, Chloride transporter ClC-3

Gene Name

Clcn3

NCBI Gene ID

UniProt

P51792

Accession Number

NP_445815.2

Cellular Locus

[Isoform 1]: Lysosome membrane, Late endosome membrane, Cell membrane, Early endosome membrane

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide correspond- ing to aa 98-115 (cytoplasmic N-terminus) of rat Clcn3 (accession no. NP_445815.2).

Target Antigen

H(+)/Cl(-) exchange transporter 3

Target

Chloride Channel Protein 3 (Clcn3)

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

[Isoform 1]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (By similarity). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity). {UniProtKB:P51790, PubMed:10915634}.; [Isoform 2]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C (PubMed:8155321). It could help neuronal cells to establish short-term memory (PubMed:8155321). {PubMed:10915634, PubMed:8155321}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~90kDa is detected. Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain membranes.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Clcn3. It does not cross-react with Clcn4 or Clcn5.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Clcn3 mediates the exchange of chloride ions against protons and functions as an antiporter, contributing to the acidification of the endosome and synaptic vesicle lumen. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.