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3X FLAG peptide (TFA)

3X FLAG peptide TFA is a FLAG-tagged peptide containing three repeats of the Asp-Tyr-Lys-Xaa-Xaa-Asp motif. 3X FLAG peptide TFA can be used for protein separation and purification, and competitive elution with target proteins.

Product Specifications

UNSPSC

12352209

Target

Others

Type

Peptides

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/3x-flag-peptide-tfa.html

Purity

99.79

Solubility

H2O : 50 mg/mL (ultrasonic)

Smiles

OC(C(F)(F)F)=O.OC(C=C1)=CC=C1C[C@H](NC([C@H](CC(O)=O)NC([C@H]([C@@H](C)CC)NC([C@H](CC(O)=O)NC([C@@H](NC([C@H](CC(O)=O)NC([C@H](CCCCN)NC([C@@H](NC([C@H](CC(O)=O)NC(CNC([C@H](CC(O)=O)NC([C@@H](NC([C@H](CC(O)=O)NC([C@H](CCCCN)NC([C@@H](NC([C@H](CC(O)=O)NC([C@@H](N)CCSC)=O)=O)CC2=CC=C(C=C2)O)=O)=O)=O)CC3=CN=CN3)=O)=O)=O)=O)CC4=CC=C(C=C4)O)=O)=O)=O)CC5=CN=CN5)=O)=O)=O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)=O

Molecular Formula

C122H170F3N31O51S

Molecular Weight

2975.84

References & Citations

[1]Zilio N, et al. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2017 Mar 1;2017 (3) :pdb.prot091611.|[2]Gao XK, et al. Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes. Cell Discov. 2022 Jun 28;8 (1) :60. |[3]Cong M, et al. MTSS1 suppresses mammary tumor-initiating cells by enhancing RBCK1-mediated p65 ubiquitination. Nat Cancer. 2020 Feb;1 (2) :222-234.

Shipping Conditions

Blue Ice

Storage Conditions

-80°C, 2 years; -20°C, 1 year (Powder, sealed storage, away from moisture)

Scientific Category

Peptides

Clinical Information

No Development Reported

Citation 01

BioRxiv. 2023 Nov 4.|Cell Death Dis. 2025 Aug 7;16 (1) :595.|Int J Mol Sci. 2025 Mar 29;26 (7) :3159.|J Adv Res. 2025 Aug:74:225-236.|Nat Microbiol. 2021 May;6 (5) :658-671.|bioRxiv. 2025 April 28.|bioRxiv. 2025 March 25.|bioRxiv. 2025 Sep 7.|Cell Discov. 2022 Jun 28;8 (1) :60.|Cell Discov. 2023 Aug 1;9 (1) :83.|Cell Res. 2025 Jun;35 (6) :437-452.|Complutense University of Madrid. 2025.|Int J Biol Macromol. 2025 Jan 7:139619.|J Biol Chem. 2024 Jul 14:107575.|J Integr Plant Biol. 2022 Sep;64 (9) :1789-1802.|J Mol Biol. 2024 Jun 20:168672.|Nat Commun. 2025 Sep 2;16 (1) :8182.|Nature Cancer. 222-234 (2020) .|Res Sq. 2025 Aug 31.|Technical University of Darmstadt. 2025 Oct 8.

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