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Anti-Integrin -beta3 (Phospho-Tyr773) Antibody

Rabbit Polyclonal Antibody specific to Integrin -beta3 (Phospho-Tyr773)

Product Specifications

Product Name Alternative

Anti-Integrin -beta3 (Phospho-Tyr773) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of tyrosine 773 (P-L-Y(p)-K-E) derived from human Integrin b3.

Target

Integrin -beta3 (Phospho-Tyr773)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~110kDa is detected. <br><br>Immunohistochemistry: use at dilution of 1:50- 1:100. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Product Datasheet

https://www.qedbio.com/wp-content/uploads/pdf/43060.pdf

Specificity

This antibody detects endogenous human, mouse, and rat Integrin b3 only when phosphorylated at tyrosine 773.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

The Integrin beta3 (ITGB3) gene encodes glycoprotein IIIa (GP IIIa), the beta subunit of the platelet membrane adhesive protein receptor complex GP IIb/IIIa. The alpha subunit, GP IIb, is encoded by the ITGA2B gene. The GP IIb/IIIa complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. Glycoprotein IIIa is also the beta subunit of 2 other integrins, fibronectin receptor and vitronectin receptor which have distinctive alpha subunits.

CAS Number

9007-83-4

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