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HRP-conjugated beta actin Monoclonal Antibody

Product Specifications

Background

Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.

Abbreviation

Beta actin

UniProt

P60709

Host

Mouse

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant Human Beta actin protein by E. Coli

Target

ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A

Clonality

Monoclonal

Clone

3A9

Conjugation

HRP

Applications

WB

Field of Research

Signal Transduction; Tags and Cell Markers; Isotype/Loading; Controls; Neuroscience; Biochemicals; Cancer; Cardiovascular; Cell Biology; Immunology; Metabolism; Epigenetics and Nuclear Signaling; Stem Cells

Purification

Protein A/G Purification

Concentration

1 mg/mL

Dilution

WB 1:2500-1:5000

Buffer

PBS with 0.1% procline 300,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Calculated Molecular Weight

43kDa

Observed Molecular Weight

43kDa

Isotype

IgG1

Available Sizes

Frequently Asked Questions

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