Anti-DFF (NT) Antibody

Rabbit Polyclonal Antibody specific to DFF (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD

Gene Name

DFFA

Gene ID

1676

NCBI Gene ID

UniProt

O00273

Accession Number

NP_004392

Cellular Locus

Cytoplasm.

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 2-21 at the N-terminus of human DFF45 (accession no. NP_004392).

Target Antigen

DNA fragmentation factor subunit α

Target

DFF (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Inhibitor of the caspase-activated DNase (DFF40).

Additionnal Information

Immunoblotting: use at 2ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from HeLa cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes non-cleaved (45kDa) and cleaved DFF.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A human DNA fragmentation factor (DFF) which is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death.