Biotinylated Onchocerca volvulus OV16 protein, Avi, GST Tag

Biotinylated Onchocerca volvulus OV16 protein, Avi, GST Tag (OV6-V81G9) is expressed from E. coli cells. It contains AA Lys 17 - Asp 197 (Accession # P31729-1) .

Product Specifications

Background

The OV16 antigen, localizes to the hypodermis, cuticle, and uterus of female O. volvulus is regarded as a highly sensitive and specific antigen for detection of IgG4 in people with prior exposure to onchocerciasis. It has an open reading frame that encodes a 152- amino acid polypeptide containing a 16-amino acid putative signal peptide. Enzyme-linked Immunosorbent Assay serological testing of OV-16 antigen is a diagnostic tool for determining effective elimination of the parasite.

Specifications

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a GST tag. The protein has a calculated MW of 48.6 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE) .

Accession Number

P31729-1

Expression Region

Lys 17 - Asp 197

Host

E. coli

Target

OV16 (Onchocerca volvulus)

Conjugation

Biotin-labeled

Tag

N-Avi & N-GST

Source

Onchocerca volvulus

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

48.6 kDa

Shipping Conditions

Storage Conditions

-20°C

Package Size

200ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/OV6-V81G9.pdf

Species