Sheep anti Human p53
Product Specifications
Background
P53 is a transcription factor with a molecular weight of 53kDa that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in Human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein.
UniProt
P04637
Host
Sheep
Species Reactivity
Human,Mouse,Rat
Isotype
IgG
Conjugation
Unconjugated
Type
Polyclonal Antibody
Source
Sheep were immunized with a GST-p53 fusion protein containing amino acids 1-393 of Human p53
Applications
WB, IP, IHC
Field of Research
Cell Cycle, Apoptosis, Cancer
Purification Method
Ammonium Sulfate Precipitation
Assay Principle
Applications: Western Blot (1-10 µg/mL), Immunoprecipitation, immunostaining of formalin-fixed and paraffine embedded tissues.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
Shipping Conditions
Ambient Temperature, freeze upon arrival
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
WB
CAS Number
9007-83-4
Curated Selection
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