Sheep anti Human p53

Product Specifications

Background

P53 is a transcription factor with a molecular weight of 53kDa that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in Human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein.

CAS Number

9007-83-4

UniProt

P04637

Host

Sheep

Species Reactivity

Human,Mouse,Rat

Isotype

IgG

Conjugation

Unconjugated

Type

Polyclonal Antibody

Source

Sheep were immunized with a GST-p53 fusion protein containing amino acids 1-393 of Human p53

Applications

WB, IP, IHC

Field of Research

Cell Cycle, Apoptosis, Cancer

Purification Method

Ammonium Sulfate Precipitation

Assay Principle

Applications: Western Blot (1-10 µg/mL), Immunoprecipitation, immunostaining of formalin-fixed and paraffine embedded tissues.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.