CheKine™ Mirco Soluble Starch Synthase (SSS) Activity Assay Kit

Soluble starch synthase (SSS, EC2.4.1.21) exists in the starch body in a free state, catalyze the starch chain elongation reaction, and is mainly responsible for the synthesis of amylopectin. CheKine™ Micro Soluble Starch Synthase (SSS) Activity Assay Kit can detect plant tissues. In this kit, SSS catalyzes the reaction between ADPG and starch primers (glucan), transfers glucose molecules to starch primers, and generates ADP at the same time. Furthermore, the addition of pyruvate kinase, hexokinase and glucose 6-phosphate dehydrogenase in the reaction system catalyzed the reduction of NADP+to NADPH in turn, where the amount of NADPH generated was proportional to the amount of ADP generated in the previous reaction. SSS activity could be calculated by measuring the increase of NADPH at 340 nm.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.