Anti-Hsp70 Antibody

Mouse Monoclonal Antibody specific to Hsp70

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Hsp70 Mouse Monoclonal Antibody

Gene ID

3303

UniProt

P08107

Accession Number

NP_005336.3

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit, Monkey, Guinea Pig, Hamster, Chicken, Bovine, Canine, Ovine, Porcine, Fish, Drosophila, C. Elegans

Immunogen

Human Hsp70 purified from HeLa cells.

Target

Hsp70

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, ELISA, FCM, FACS, IEM, Bl, AM

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: 1ug/mL. A band of 70kDa is detected.Immunoblotting of Hsp70 in cell lysates from human cancer cell lines with #11096. Immunohistochemistry: 1-10ug/mLImmunohistochemical staining of inflammatory cells in human colon tissue with #11096. Immunoprecipitation: 1-10ug/ mLELISA: 1-10ug/mLFlow cytometry: 10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes Hsp70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, and C. elegans and does not cross-react with Hsc70. The epitope is in the region of amino acids 436- 503 of human Hsp70.

Formulation

PBS containing 0.1% sodium azide

Target Background

Hsp70 genes, members of a multigene family, encode heat-inducible 70kDa heat- shock proteins that function as molecular chaperones. They have been identified in most organelles of eukaryotic cells as well as in bacteria. The N-terminus of Hsp70 binds ATP with high affinity and the C- terminus binds proteins and polypeptides. Hsp70s bind nascent polypeptides as well as partially folded intermediates of proteins, thus preventing their aggregation and/or misfolding. Binding of ATP triggers a conformational change that results in the release of bound protein. Hsp70s function in protein synthesis, protein folding and oligomerization, and protein transport.