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Recombinant Mouse Csf3 Protein (Trx Tag)

Product Specifications

Background

Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to the IL-6 superfamily. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent.

Abbreviation

Csf3

Synonyms

CSF; TNFSF; C17orf; C17orf33; CD254; CSF3OS; Filgrastim; GCSF; G-CSF; Granulocyte colony-stimulating factor; Lenograstim; ODF (Osteoclast Differentiation Factor) ; OPGL; Pluripoietin; soluble Receptor Activator of NF-kB Ligand; sRNAK Ligand; TNFSF11; TRANCE (TNF-Related Activation-induced Cytokine) ; Csfg; Csf3

UniProt

P09920

Accession Number

P09920

Expression System

E.coli

Tag

N-Trx

Sequence

Val31-Ala208

Field of Research

Immunology; Cancer; Cell Biology; Signal Transduction; Stem cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.6 kDa

Observed Molecular Weight

35 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse

Available Sizes

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