Anti-ASIC1 Antibody

Mouse Monoclonal Antibody specific to AISC1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

ASIC1, Acid-sensing ion channel, Amiloride-sensitive cation channel 2, neuronal, Brain sodium channel 2, BNaC2

Gene Name

Asic1

NCBI Gene ID

UniProt

Q6NXK8

Accession Number

NP_033727.1

Cellular Locus

Cell membrane

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Fusion protein corresponding to aa 460-526 (cytoplasmic C-terminus) of mouse ASIC1 (accession no. Q6NXK8).

Target Antigen

Acid-sensing ion channel 1

Target

ASIC1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Proton-gated sodium channel; it is activated by a drop of the extracellular pH and then bPubMed:11988176, PubMed:12843249, PubMed:15369669, PubMed:15578512, PubMed:17060608, PubMed:17662962}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~60kDa is detected. <br><br>Positive control: Rat brain lysate. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse and rat ASIC1. It does not cross-react with ASIC2.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Acid sensing (proton-gated) ion channel 1 (ASIC1) is a cation channel with high affinity for sodium which is gated by extracellular protons and inhibited by the diuretic amiloride. It is also permeable for Ca(2+), Li(+) and K(+) and mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. ASIC1 functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin- dependent protein kinase II phosphory- lation.