Nuclear Protein Isolation-Translocation Kit

PARTITION & CONCENTRATE nuclear from cytoplasmic proteins in 45 min for EMSA, Western blot & translocation assays using cells or tissues.  For 300+ isolations based on one well in a 6-well dishes, or 100 isolations using 10 cm dishes.  Also applicable for tissues.  This nuclear protein extraction kit partitions and concentrates proteins in nuclei and cytosol in a very simple 45 minute protocol using a microcentrifuge.  Simplicity:  Allows for simultaneous isolations of multiple samples. Scalability:  Suitable for 300+ isolations/extractions based on a well in a 6-well dish, or 100 isolations/extractions using semi-confluent 10 cm dishes. Versibility: Applicalbe for cells from virtually any mammalian species, or with tissues using an initial homogenization step. Broad Downstream Applicability:  Ideal for nuclei isolation, nuclei-cytosol fractionation, nuclear protein extraction, measurement of nuclear import, EMSA, transcription factor activation and translocation assays.