Colistin ELISA Kit

Colistin is a polymyxin antibiotic that is used as the last line of treatment for infections caused by multi-drug resistant organisms such as Pseudomonas, Klebsiella, and Escherichia species. The drug has been demonstrated to cause nephrotoxicity and neurotoxicity; however, the toxicity is dose-dependent and can be reversed by discontinuation of the treatment. The antibiotic is also used as a food additive in the animal farming industry, especially pig, cow, and rabbit farms to promote growth and treat intestinal infections caused by multidrug-resistant organisms. The drug is also administered with feed, milk, and water. BioVision’s Colistin ELISA kit is used to quantitatively measure Colistin in muscle tissue and urine samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Colistin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Colistin in the samples.