Human ACE2 / ACEH Protein, Mouse IgG1 Fc Tag (MALS verified)

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

Product Specifications

Background

Specifications

This protein carries a mouse IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 109.8 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q9BYF1-1

Expression Region

Gln 18 - Ser 740

Host

HEK293

Target

ACE2

Conjugation

Unconjugated

Tag

C-mFc (mIgG1)

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Human ACE2, Mouse IgG1 Fc Tag (Cat. No. AC2-H5205) with a linear range of 0.4-6 ng/mL (QC tested).